Transfer membrane is widely used in the process of transfering and detection of proteins and nucleic acids. In order to achieve the best test result, it is pivotal to choose the right ,uniform and stable membrane.
Choice of the transfer pmembrane : the ability of conbine protein directly effect the transfer result; According to the different molecular weight of proteins, choose appropriate pore size to ensure that the filter paper, transfer membrane,and gel closely contact with each other without bubbles
Low transfer temperature , better transfer result
Transfer time : big protein weight lead to longer transfer time ,pay attention to the problem of over transfer.
Transfer voltage or current, buffer formula, ph value, also affect transfer efficiency
▶ Cobetter nitrocellulose membrane
